BIOTIN MODIFIER, STREPTAVIDIN MUTANT, AND UTILIZATION THEREOF

PROBLEM TO BE SOLVED: To provide a streptavidin mutant having reduced affinity to natural biotin, and to provide a biotin modifier having high affinity to the streptavidin mutant having low affinity to the natural biotin.SOLUTION: There is provided a reagent kit for treatment or diagnosis containing...

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Hauptverfasser:	IIZUKA TOMOHIRO, KAWATO TATSUYA, SUGIYAMA AKIRA, DOI HIROFUMI, INOUE TAKESHI, MIZOHATA EIICHI, YAMAMOTO TOMOHIRO, KODAMA TATSUHIKO, KANAI MOTOMU, SHIMIZU YOHEI
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a streptavidin mutant having reduced affinity to natural biotin, and to provide a biotin modifier having high affinity to the streptavidin mutant having low affinity to the natural biotin.SOLUTION: There is provided a reagent kit for treatment or diagnosis containing (a) a streptavidin mutant having reduced affinity to natural biotin or biocytin, and (b) a biotin modifier having high affinity to the streptavidin mutant.SELECTED DRAWING: None 【課題】天然ビオチンに対する親和性を低減させたストレプトアビジン変異体を提供し、更にこの天然ビオチンに対する低親和性のストレプトアビジン変異体に対して高い親和性を有するビオチン改変体を提供すること。【解決手段】（ａ）天然ビオチン又はビオシチンとの親和性を低下させたストレプトアビジン変異体、及び；（ｂ）上記ストレプトアビジン変異体に対して高い親和性を有するビオチン改変体：を含む、治療又は診断のための試薬キット。【選択図】なし