PROKARYOTIC EXPRESSION CONSTRUCT

PROBLEM TO BE SOLVED: To provide an expression construct for producing a polypeptide in a prokaryotic cell, comprising a pro-polypeptide comprising, in the N- to C-terminal direction, a dipeptide GS, an amino acid tag, a dipeptide GS and a protease cleavage site.SOLUTION: A pro-polypeptide comprises...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ADELBERT GROSSMANN, WILMA LAU, FREDERIKE HESSE, CHRISTIAN SCHANTZ, KOPETZKY ERHARD
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide an expression construct for producing a polypeptide in a prokaryotic cell, comprising a pro-polypeptide comprising, in the N- to C-terminal direction, a dipeptide GS, an amino acid tag, a dipeptide GS and a protease cleavage site.SOLUTION: A pro-polypeptide comprises in the N- to C-terminal direction: a first dipeptide GS; an amino acid sequence tag; a second dipeptide GS adjacent immediately adjacent to an enzymatic cleavage site; and the enzymatic cleavage site.SELECTED DRAWING: None 【課題】Ｎ末端からＣ末端の方向に、ジペプチドＧＳ、アミノ酸タグ、ジペプチドＧＳおよびプロテアーゼ開裂部位を含むプロポリペプチドを含む、原核細胞におけるポリペプチドの産生のための発現構築物の提供。【解決手段】Ｎ末端からＣ末端の方向に、−第１のジペプチドＧＳ、−アミノ酸配列タグ、−以下にすぐに隣接した第２のジペプチドＧＳ、−酵素的開裂部位を含む、プロポリペプチド。【選択図】なし