MODIFIED PYRANOSE OXIDASES

PROBLEM TO BE SOLVED: To provide modified pyranose oxidases unsusceptible to oxygen, genes thereof, vectors, transformed cells and production methods thereof, as well as methods for measuring pyranose by using the enzyme, reagent compositions and sensors for pyranose measurement.SOLUTION: It was fou...

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Hauptverfasser:	NAKATSUKA TOMOKO, ARAKI TOSHIO
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide modified pyranose oxidases unsusceptible to oxygen, genes thereof, vectors, transformed cells and production methods thereof, as well as methods for measuring pyranose by using the enzyme, reagent compositions and sensors for pyranose measurement.SOLUTION: It was found that modified pyranose oxidases having an excellent feature that being unsusceptible to oxygen can be obtained by substituting amino acids of a pyranose oxidase (POD). Further, it was found that by using a polynucleotide encoding the modified enzyme, the enzyme can be produced with efficiency, and that pyranose measurement unsusceptible to oxygen is enabled by using the enzyme of the invention. 【課題】酸素の影響を受けにくい改変型ピラノース酸化酵素、該酵素遺伝子、ベクター、形質転換細胞及び該酵素の製造方法を提供する。更に、該酵素によるピラノースの測定方法、測定試薬組成物及びセンサを提供するものである。【解決手段】ピラノース酸化酵素（ＰＯＤ）のアミノ酸を置換することで、酸素の影響を受けにくいという優れた特性を有する改変型ピラノース酸化酵素を取得できることを見出した。更に、該酵素をコードするポリヌクレオチドを用いて該酵素を効率的に製造できるということ、及び該酵素を用いて酸素の影響を受けにくいピラノース測定が可能なことを見出し、本発明に至った。【選択図】図１