DRY POWDER CELLS AND CELL CULTURE REAGENTS AND METHODS OF PRODUCTION THEREOF
PROBLEM TO BE SOLVED: To provide nutritive (e.g., cell culture) medium, medium supplement, medium subgroup and buffer formulations, particularly, powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such...
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Format: | Patent |
Sprache: | eng ; jpn |
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Zusammenfassung: | PROBLEM TO BE SOLVED: To provide nutritive (e.g., cell culture) medium, medium supplement, medium subgroup and buffer formulations, particularly, powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells.SOLUTION: The invention provides powdered formulations that produce particular or desired final ionic and/or pH conditions in reconstitution with a solvent. The invention is intended to provide methods of production of these formulations, and also kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods.
【課題】栄養(例えば細胞培養)培地、培地補足物、培地部分群および緩衝液の処方に広く関連する。特に、粉末栄養培地、培地補足物および培地部分群の処方、特に細胞培養培地補足物(粉末ウシ胎児血清(FBS)のような粉末血清を含む)、培地部分群の処方、および、細胞のインビトロの培養を促進する必須栄養素を含む細胞培養培地を提供する。【解決手段】溶媒による再構成の際に、特定のまたは望ましい最終イオン条件および/またはpH条件を生み出すような粉末処方。これらの処方の生産方法を意図し、これらの処方を用いた原核細胞および真核細胞の培養のためのキットおよび方法。滅菌処方を生産する方法、および、乾燥細胞粉末を生産するための方法。本方法によって生産される、細胞、培地、培地補足物、培地部分群および緩衝液の粉末。【選択図】なし |
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