METHOD TO OBTAIN OSTEOBLAST OR CELL CAPABLE OF DIFFERENTIATING INTO OSTEOBLAST, CAPABLE OF ANALYZING CELL SURFACE MARKER BY FLOW CYTOMETRY

PROBLEM TO BE SOLVED: To provide a method to obtain osteoblasts or cells capable of differentiating into osteoblasts, capable of analyzing a cell surface marker by flow cytometry.SOLUTION: A method for removing calcification material from osteoblasts or cells capable of differentiating into osteobla...

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Bibliographische Detailangaben
Hauptverfasser:	YAMAMOTO YUMIKO, HAYASHI MIKAKO, ITO SHOSAKU
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method to obtain osteoblasts or cells capable of differentiating into osteoblasts, capable of analyzing a cell surface marker by flow cytometry.SOLUTION: A method for removing calcification material from osteoblasts or cells capable of differentiating into osteoblasts, includes a step in which two kinds of density gradient medium liquids with different concentrations are superposed, and further the osteoblasts or cells capable of differentiating into osteoblasts are superposed on an upper layer thereof for centrifugation. 【課題】本発明は、細胞表面マーカーをフローサイトメトリーで分析可能な骨芽細胞又は骨芽細胞に分化し得る細胞を得る方法を提供することを目的とする。【解決手段】骨芽細胞又は骨芽細胞に分化し得る細胞から石灰化物を除去する方法であって、２種類の濃度の異なる密度勾配媒体液を重層し、さらにその上層に骨芽細胞又は骨芽細胞に分化し得る細胞を重層して遠心分離する工程を含むことを特徴とする方法。【選択図】なし