PURIFICATION METHOD OF VIRUS-LIKE PARTICLES

PROBLEM TO BE SOLVED: To provide a novel purification method of norovirus virus-like particles.SOLUTION: A purification method of norovirus virus-like particles includes steps of: contacting a solution containing the norovirus virus-like particles with a carrier for hydroxyapatite chromatography to...

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Hauptverfasser:	OKADA KIYOHIRO, MUKAI AKIKO, ITO HIROYUKI, NISHINO TOMONORI, SATO MAMORU, ARINOBU DAISUKE
Format:	Patent
Sprache:	eng ; jpn
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a novel purification method of norovirus virus-like particles.SOLUTION: A purification method of norovirus virus-like particles includes steps of: contacting a solution containing the norovirus virus-like particles with a carrier for hydroxyapatite chromatography to bind the virus-like particles to the carrier; subsequently, washing the carrier with a buffer; and then, eluting the virus-like particles from the carrier with a buffer containing a phosphate, the phosphate level of the buffer used for the elution being less than 10 mM. 【課題】ノロウイルスのウイルス様粒子の新規な精製手段を提供する。【解決手段】ノロウイルスのウイルス様粒子を含む溶液を、ハイドロキシアパタイトクロマトグラフィー用担体と接触させ、前記ウイルス様粒子を前記担体に結合させ、その後、バッファで前記担体を洗浄し、次いで、リン酸塩を含むバッファで前記担体から前記ウイルス様粒子を溶出させることにより、前記ウイルス様粒子を精製する方法であって、前記溶出に用いたバッファのリン酸塩濃度が10mM未満であることを特徴とするノロウイルスのウイルス様粒子の精製方法。【選択図】なし