PLASTIC MICROFLUIDIC SEPARATION AND DETECTION PLATFORMS

PROBLEM TO BE SOLVED: To provide inexpensive, multi-lane plastic biochips capable of performing focused nucleic acid analysis at high resolution and with a high signal to noise ratio, and methods of using such chips.SOLUTION: Plastic electrophoresis separation chips 100 are provided comprising a plurality of microfluidic channels 106 and a detection window 107, where the detection window 107 comprises a thin plastic, and the detection window 107 comprises a detection region of each microfluidic channel 106. Such chips 100 can be bonded to a support, and an aperture 107 is provided in the support to allow laser induced fluorescence detection of samples in the electrophoresis chip 100 at the thin plastic detection window 107. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip 100 are described. 【課題】高分解能にて集中核酸分析を行うことができ、高い信号対雑音比を持つ安価な複数レーンのプラスチック・バイオチップ、並びにかかるチップを用いる方法の提供。【解決手段】複数のマイクロ流体チャネル１０６及びある検出窓１０７を含み、検出窓１０７は薄いプラスチックを含み、検出窓１０７は、各マイクロ流体チャネル１０６の検出領域を含む、プラスチック電気泳動分離チップ１００。かかるチップ１００は支持体に結合でき、開口部１０７を支持体に設けて、薄いプラスチック検出窓１０７で電気泳動チップ１００中の試料のレーザー誘起蛍光の検出を可能にする。更に、プラスチック電気泳動分離チップ１００上の複数の試料を電気泳動的に分離及び検出する方法。【選択図】図１