WHOLE CELL BIOCATALYSTS FOR THE DEGRADATION OF CELLULOSIC BIOMASS

PROBLEM TO BE SOLVED: To provide a microorganism expressing on its surface a cellulase that is sufficiently stable under the reaction conditions for degradation of cellulosic biomass.SOLUTION: Provided is a nucleic acid molecule comprising: (1) a portion encoding signal peptide; (2) a portion encodi...

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Bibliographische Detailangaben
Hauptverfasser:	TATJANA BROSSETTE, RUTH MAAS, ANDREAS ROHARDT, MICHAEL JUERGENS
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a microorganism expressing on its surface a cellulase that is sufficiently stable under the reaction conditions for degradation of cellulosic biomass.SOLUTION: Provided is a nucleic acid molecule comprising: (1) a portion encoding signal peptide; (2) a portion encoding heterogeneous cellulase; (3) optionally a portion encoding protease-recognizing site; (4) a portion encoding a transmembrane linker; and (5) a portion encoding a transporter domain of an autotransporter or variants thereof. Also provided is a nucleic acid molecule encoding a cellulase selected from the group consisting of -glucosidases and endo- or exo-cellulases. Also provided is a nucleic acid molecule comprising an expression control sequence which, preferably, is functionally linked to the nucleic acid and can be activated by addition of isopropyl thioglucopyranoside (IPTG) or arabinose.