100% SEQUENCE IDENTITY DETECTION METHODS FOR VARIABLE GENOMES

PROBLEM TO BE SOLVED: To provide methods, reagents and kits for detection of all known human variants of influenza A virus and at least 90% of avian and swine variants of influenza A virus in biological samples.SOLUTION: A set of polynucleotides capable of amplifying at least 90% of human, swine and...

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1. Verfasser:	SAGHBINI MICHAEL G
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide methods, reagents and kits for detection of all known human variants of influenza A virus and at least 90% of avian and swine variants of influenza A virus in biological samples.SOLUTION: A set of polynucleotides capable of amplifying at least 90% of human, swine and avian variants of influenza A virus comprises at least one primer pair comprising: a forward primer comprising at least 10 consecutive nucleotides of a particular base sequence or a complementary strand thereof, and a reverse primer comprising at least 10 consecutive nucleotides of a particular sequence or a complementary strand thereof. A kit for amplifying influenza A virus variants comprises a pair of primers comprising a forward primer and a reverse primer. There is provided a method of detecting the presence of at least 90% of variants of influenza A virus.