METHOD FOR PREPARING SAMPLE CONTAINING ONE MOLECULE OF DNA HAVING TARGET BASE SEQUENCE

PROBLEM TO BE SOLVED: To provide a method capable of preparing a DNA solution sample containing one molecule of a target base sequence.SOLUTION: A base sequence different from a target base sequence is detected by a real-time PCR on a DNA molecule a part of which has a target base sequence, and base...

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Hauptverfasser: YOSHII JUNJI, NUNOFUJI SATOSHI, KITSUTA KAZUMI, MANO JUNICHI, TAKABATAKE REONA, NAKAE HIROKI
Format: Patent
Sprache:eng
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Zusammenfassung:PROBLEM TO BE SOLVED: To provide a method capable of preparing a DNA solution sample containing one molecule of a target base sequence.SOLUTION: A base sequence different from a target base sequence is detected by a real-time PCR on a DNA molecule a part of which has a target base sequence, and based on a detection result of the real-time PCR, a solution sample containing one molecule of the DNA molecule is selected from a group of solution samples in which the DNA molecule is contained at the concentration of one molecule or less in average per one sample.