METHOD FOR IMPROVING ENZYME TO BE USED FOR BIOSYNTHESIS OF 1,4-BUTANEDIOL IN E. COLI, AND METHOD FOR SCREENING MODIFIED GENES

METHOD FOR IMPROVING ENZYME TO BE USED FOR BIOSYNTHESIS OF 1,4-BUTANEDIOL IN E. COLI, AND METHOD FOR SCREENING MODIFIED GENES

PROBLEM TO BE SOLVED: To provide a method for modifying enzymes to be used in biosynthesis of 1,4-butanediol (1,4-BDO) in E.coli, and a method for screening the modified genes.SOLUTION: Improved microorganism was prepared by introducing and expressing butyraldehyde dehydrogenase and butanol dehydrog...

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Bibliographische Detailangaben
Hauptverfasser: LEE YOUNG-MIN, PARK JAEAN, LEE PYUNGON, PARK JIN-HWAN, LEE WOO-YONG
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Zusammenfassung:PROBLEM TO BE SOLVED: To provide a method for modifying enzymes to be used in biosynthesis of 1,4-butanediol (1,4-BDO) in E.coli, and a method for screening the modified genes.SOLUTION: Improved microorganism was prepared by introducing and expressing butyraldehyde dehydrogenase and butanol dehydrogenase in E. Coli, where these enzymes are derived from Clostridium saccharoperbutylacetonicum, and their activities in the two terminal reactions in the 1,4-BDO biosynthesis pathway have not been known, that is, in the formation reaction of 4-hydroxybutyraldehyde from 4-hydroxybutyryl-CoA, and in the formation reaction of 1,4-BDO from 4-hydroxybutyraldehyde respectively. As a result, the transformed E.coli was confirmed to have 1,4-BDO productivity. When the activity of butyraldehyde dehydrogenase was strengthened by the directed evolution, the product concentration of 1,4-BDO was increased twice or more.