POSITIVE CONTROL CONCEPT

PROBLEM TO BE SOLVED: To provide methods for detecting or quantifying at least two different target nucleic acids in separate vials for detecting and quantifying the target nucleic acids by amplification.SOLUTION: Methods are provided for detecting or quantifying at least two different target nuclei...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: RIEDLING MICHAEL, KUEHLER LEIF, SIMON CHRISTIAN, ZIMMERMANN DIRK, NAMASIVAYAM VIJAY, ROEHRIG SASCHA
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:PROBLEM TO BE SOLVED: To provide methods for detecting or quantifying at least two different target nucleic acids in separate vials for detecting and quantifying the target nucleic acids by amplification.SOLUTION: Methods are provided for detecting or quantifying at least two different target nucleic acids using a single positive control stock solution comprising a mixture of positive control nucleic acids for the different target nucleic acids to be detected or quantified, and the method is performed in an automated analyzer. Kits and its use are also provided. The kit comprises a rack comprising at least two positive control vials, wherein each of the positive control vials comprises a stock solution of a mixture of at least two positive control nucleic acids, and a label indicating a single target nucleic acid to be detected with the components.