ACRYLAMIDE-DEGRADING SELF-CLONING ASPERGILLUS ORYZAE

PROBLEM TO BE SOLVED: To provide a self-cloning Aspergillus oryzae expressing an amidase without using induction culture and having high acrylamide degrading activity and a method for reducing acrylamide by using the Aspergillus oryzae.SOLUTION: There are provided a self-cloning Aspergillus oryzae t...

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Hauptverfasser:	FUKUNAGA TAIJI, NARITA YUSAKU, SANO MOTOAKI, OZEKI KENJI, IWAI KAZUYA, TSUBOI HIROKAZU, BOGAKI TAKAYUKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COFFEE COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES MANUFACTURE, PREPARATION, OR INFUSION THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TEA THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR SUBSTITUTES THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a self-cloning Aspergillus oryzae expressing an amidase without using induction culture and having high acrylamide degrading activity and a method for reducing acrylamide by using the Aspergillus oryzae.SOLUTION: There are provided a self-cloning Aspergillus oryzae that is transfected in a state that expresses, without induction culturing, a gene encoding a polypeptide having a specific amino acid sequence derived from the Aspergillus oryzae or a gene having a sequence hybridizable under stringent conditions with a nucleic acid molecule comprising a base sequence complementary to a nucleic acid molecule encoding the polypeptide and further encoding a protein having amidase activity; a method for reducing acrylamide comprising a step of treating acrylamide-containing matter with contact with the Aspergillus oryzae; and a method for producing a reduced-acrylamide food or beverage.