METHOD

PROBLEM TO BE SOLVED: To provide a purification method for a highly pure albumin, comprising a step of obtaining a purified albumin solution by applying a first albumin solution in a range of pH of 8.0-9.5 and a conductivity of 1-75 mS.cmto an affinity chromatography step of performing a negative mo...

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Bibliographische Detailangaben
Hauptverfasser:	CARTWRIGHT ANDREW JOHN, VERON JEAN-LUC BERNARD, WOODROW JOHN RODNEY, MEAD DAVID JOHN, VAN URK HENDRIK, BALLANCE DAVID JAMES, BEREZENKO STEPHEN, MORTON PHILIP HARVEY, GRANDGEORGE MICHEL GASTON JOSEPH, SLEEP DARRELL, CAMERON JASON
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a purification method for a highly pure albumin, comprising a step of obtaining a purified albumin solution by applying a first albumin solution in a range of pH of 8.0-9.5 and a conductivity of 1-75 mS.cmto an affinity chromatography step of performing a negative mode for albumin and using an affinity matrix containing immobilized dihydroxyboryl group.SOLUTION: There is provided a production method for recombinant albumin, which includes culturing a fungal cell expressing a recombinant albumin coding sequence, wherein the cell has a reduced capacity of mannosylation of the recombinantly-expressed albumin. The purification method for high-purification-expressed and secreted albumin comprises the steps of performing positive mode cation exchange chromatography, positive mode anion exchange chromatography, positive mode affinity chromatography, negative mode affinity chromatography (preferably using immobilized aminophenylboronic acid), negative mode cation exchange chromatography, and negative or positive mode anion exchange chromatography.