ENHANCED PRODUCTION OF LIPIDS CONTAINING POLYENOIC FATTY ACIDS BY HIGH DENSITY CULTURES OF EUKARYOTIC MICROBES IN FERMENTORS

PROBLEM TO BE SOLVED: To provide a method of proliferating eukaryotic microbes capable of producing a lipid, particularly a polyenoic fatty acid-containing lipid, and to provide a method of producing eukaryotic microbial lipids.SOLUTION: There are provided the method of proliferating the eukaryotic...

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Hauptverfasser:	DIMASI DON, VEEDER GEORGE T III, KANEKO TATSUO, HANSEN JON M, BARCLAY WILLIAM R, REUCKER CRAIG M, MIRRASOUL PETER J, BAILEY RICHARD B
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL RIPENING OF FRUIT OR VEGETABLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKINGOILS ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THE PRESERVED, RIPENED, OR CANNED PRODUCTS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method of proliferating eukaryotic microbes capable of producing a lipid, particularly a polyenoic fatty acid-containing lipid, and to provide a method of producing eukaryotic microbial lipids.SOLUTION: There are provided the method of proliferating the eukaryotic microorganisms capable of producing at least about 20% of an eukaryotic microorganisms biomass as lipids and the method of producing the lipids. The lipids preferably contain one or more polyenoic fatty acids. A carbon source, preferably a non-alcohol carbon source and a restricted nutrient source are added to a fermentation medium containing the eukaryotic microorganisms. The carbon source and the restricted nutrient source are preferably added at a rate sufficiently enough to increase the biomass density of the fermentation medium to at least about 100 g/L.