PLASMID-TYPE VECTOR, METHOD FOR DETECTING GENE PROMOTER ACTIVITY AND ASSAY KIT

PROBLEM TO BE SOLVED: To detect activity of a gene promoter in high sensitivity.SOLUTION: The plasmid-type vector 1 has a gene promoter 10. A reporter gene 21, an internal ribosome entry site (IRES) 23, a replication initiation protein gene 25, and a transcription termination signal sequence 27 are...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	AKABOSHI HIDEKAZU, ISHIHARA MITSUKO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING ELECTRIC VARIABLES MEASURING MAGNETIC VARIABLES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To detect activity of a gene promoter in high sensitivity.SOLUTION: The plasmid-type vector 1 has a gene promoter 10. A reporter gene 21, an internal ribosome entry site (IRES) 23, a replication initiation protein gene 25, and a transcription termination signal sequence 27 are arranged at the downstream of the gene promoter 10. The reporter gene 21 encodes a reporter protein for visualizing activity of the gene promoter 10. The replication initiation protein gene 25 encodes the replication initiation protein. The IRES 23 is arranged between the reporter gene 21 and the replication initiation protein gene 25. The transcription termination signal sequence 27 encodes a signal for terminating transcription of the reporter gene 21 and the replication initiation protein gene 25. The plasmid-type vector 1 has a replication initiation sequence 30. The replication initiation sequence 30 is recognized by the replication initiation protein.