METHOD FOR CONCENTRATING CELL SUSPENSION

PROBLEM TO BE SOLVED: To process a large amount of cell suspension simply and quickly.SOLUTION: This method for concentrating cells, by introducing the cell suspension into a container having an inlet port and an outlet port, and filled with a filter obtained by laminating nonwoven fabrics, introduc...

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1. Verfasser: TANIGUCHI SHUHEI
Format: Patent
Sprache:eng
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Zusammenfassung:PROBLEM TO BE SOLVED: To process a large amount of cell suspension simply and quickly.SOLUTION: This method for concentrating cells, by introducing the cell suspension into a container having an inlet port and an outlet port, and filled with a filter obtained by laminating nonwoven fabrics, introducing recovery solution into the container after discharging the filtrate without substantially containing the cells, and recovering the cells captured in the filter, is characterized in that (a) the mean fiber diameter of each of the layers is ≥1.5 μm, (b) at least two layers have a ≤5.0 μm mean fiber diameter, and (c) while paying attention only to the layers having a ≤5.0 μm mean fiber diameter, any two adjoining layers satisfies the relations of (c1) U>D and also U-D≥0.5, or (c2) U