PLANT TRANSFORMATION AND SELECTION

PROBLEM TO BE SOLVED: To provide a genotype-independent method for transforming and selecting plant explants.SOLUTION: The transformation method includes: a step of pre-culturing the explants in the presence of an Agrobacterium inducer; and a step of exposing the transformed explants to a shoot rege...

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Bibliographische Detailangaben
Hauptverfasser:	HANDLEY LEVIS W, THOMAS ROBERT D, CHANG SHUJUN, HAMILTON RANDY L, CONNETT MARIE B
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING IMPREGNATING OR COATING OF PAPER METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PAPER NOT OTHERWISE PROVIDED FOR PAPER-MAKING PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D PRODUCTION OF CELLULOSE PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PULP COMPOSITIONS SPIRITS TEXTILES TRAPPING TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a genotype-independent method for transforming and selecting plant explants.SOLUTION: The transformation method includes: a step of pre-culturing the explants in the presence of an Agrobacterium inducer; and a step of exposing the transformed explants to a shoot regeneration media that accelerates shoot development. There are provided plants generated from this transformation method. There are provided methods for obtaining transgenic Eucalyptus and pine cells and regenerating stably transformed Eucalyptus and pine trees. The invention also provides media, methods, and plasmids for selecting and regenerating plants, particularly forest trees.