METHOD AND COMPOSITION FOR DETERMINING PURITY OF CHEMICALLY SYNTHESIZED NUCLEIC ACID

PROBLEM TO BE SOLVED: To provide a method for separating synthetic oligonucleotides protected from fully deprotected synthetic oligonucleotides.SOLUTION: A method for separating the synthetic oligonucleotides protected from fully deprotected synthetic oligonucleotides includes: a step of bringing a...

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Hauptverfasser:	AGRIS PAUL F, PEARCE CHRISTOPHER D J, MITCHELL LLOYD G
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for separating synthetic oligonucleotides protected from fully deprotected synthetic oligonucleotides.SOLUTION: A method for separating the synthetic oligonucleotides protected from fully deprotected synthetic oligonucleotides includes: a step of bringing a mixture of protected synthetic oligonucleotides and fully deprotected synthetic oligonucleotides into contact with antibodies that specifically binds to the synthesized oligonucleotides covalently binding to an organic protecting group but not binding thereto if the organic protecting group does not covalently bind; and a step of purifying the synthesized oligonucleotides fully deprotected from the protected oligonucleotides to which the antibodies bind.