IMPROVED TYPE HALOHYDRIN EPOXIDASE

PROBLEM TO BE SOLVED: To obtain improved type halohydrin epoxidases in which stereoselectivity, product inhibition resistance, etc., are improved, and provide a method for efficiently producing an optically active epihalohydrin or a 4-halo-3-hydroxybutyronitrile by utilizing them.SOLUTION: In the am...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	KUWAHARA FUMI, TO FUJIO, AKIYAMA TAKAMICHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To obtain improved type halohydrin epoxidases in which stereoselectivity, product inhibition resistance, etc., are improved, and provide a method for efficiently producing an optically active epihalohydrin or a 4-halo-3-hydroxybutyronitrile by utilizing them.SOLUTION: In the amino acid sequence of a wild type halohydrin epoxidase consisting of a specific amino acid sequence, the improved type halohydrin epoxidases of which second Ala is substituted with Lys and introducing at least one kind of amino acid mutation selected from the following (i) to (l), are provided. (i) The amino acid mutation in which 29th Glu is substituted with Lys, (j) the amino acid mutation in which 179th Glu is substituted with Gln, (k) the amino acid mutation in which both 183th Asp and 184th Met are substituted with Val, and (l) the amino acid mutation in which 221th Phe is substituted with Leu.