MODIFIED NUCLEOTIDE AND REAL-TIME POLYMERASE REACTION USING THE SAME

PROBLEM TO BE SOLVED: To provide modified nucleotides and a real-time polymerase reaction using the same.SOLUTION: Nucleotides to which a fluorescent substance is bonded, a composition for the real-time polymerase reaction containing the nucleotides, an analysis kit, and an analysis method are provi...

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description PROBLEM TO BE SOLVED: To provide modified nucleotides and a real-time polymerase reaction using the same.SOLUTION: Nucleotides to which a fluorescent substance is bonded, a composition for the real-time polymerase reaction containing the nucleotides, an analysis kit, and an analysis method are provided. Nucleotides which are one of polymerase reaction substrates are chemically modified to impart a function of generating fluorescent signals in addition to a substrate function. Consequently, since there is no need to produce a probe, it is economical, and the nucleotides are easily applicable to various real-time polymerase reactions such as PCR (polymerase chain (amplification) reaction), RCA (rolling circle amplification) and isothermal polymerization reaction. Further, analytical performance is also excellent compared with conventional methods.
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Nucleotides which are one of polymerase reaction substrates are chemically modified to impart a function of generating fluorescent signals in addition to a substrate function. Consequently, since there is no need to produce a probe, it is economical, and the nucleotides are easily applicable to various real-time polymerase reactions such as PCR (polymerase chain (amplification) reaction), RCA (rolling circle amplification) and isothermal polymerization reaction. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title MODIFIED NUCLEOTIDE AND REAL-TIME POLYMERASE REACTION USING THE SAME
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