MODIFIED NUCLEOTIDE AND REAL-TIME POLYMERASE REACTION USING THE SAME

PROBLEM TO BE SOLVED: To provide modified nucleotides and a real-time polymerase reaction using the same.SOLUTION: Nucleotides to which a fluorescent substance is bonded, a composition for the real-time polymerase reaction containing the nucleotides, an analysis kit, and an analysis method are provi...

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1. Verfasser:	AN DAIRO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide modified nucleotides and a real-time polymerase reaction using the same.SOLUTION: Nucleotides to which a fluorescent substance is bonded, a composition for the real-time polymerase reaction containing the nucleotides, an analysis kit, and an analysis method are provided. Nucleotides which are one of polymerase reaction substrates are chemically modified to impart a function of generating fluorescent signals in addition to a substrate function. Consequently, since there is no need to produce a probe, it is economical, and the nucleotides are easily applicable to various real-time polymerase reactions such as PCR (polymerase chain (amplification) reaction), RCA (rolling circle amplification) and isothermal polymerization reaction. Further, analytical performance is also excellent compared with conventional methods.