WHOLE BLOOD PREPARATION FOR CYTOMETRIC ANALYSIS OF CELLULAR SIGNAL TRANSDUCTION PATHWAYS

PROBLEM TO BE SOLVED: To provide a method for preparing a biological sample for the measurement of protein epitopes that enables intracellular protein epitopes to be preserved and signal transduction pathways to be detected based on the ability to capture the transient active state of the epitopes.S...

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Bibliographische Detailangaben
Hauptverfasser:	DAVID HEDLEY, T VINCENT SHANKEY, PATRICIA GROM, SI CHOU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for preparing a biological sample for the measurement of protein epitopes that enables intracellular protein epitopes to be preserved and signal transduction pathways to be detected based on the ability to capture the transient active state of the epitopes.SOLUTION: The present invention enables a biological sample including erythrocytes to be fixed quickly to ensure that epitopes of signal transduction molecules and other intracellular protein epitopes are preserved in an active state. The method of the invention enables erythrocyte lysis and can be useful for the cytometric analysis of a biological sample, for example, whole blood, bone marrow aspirate, ascites, and other sample including erythrocytes. The invention also provides a method for recovering or unmasking epitopes on intracellular antigens adapted to make crosslinking fixative inapproachable which is necessary to fix the sample. The invention enables the preservation and analysis of phosphate-epitope level in a biological sample collected directly from a patient to determine disease-specific characteristics.