APPARATUS FOR SEARCHING PRIMER SET, METHOD AND PROGRAM FOR SEARCHING PRIMER SET
PROBLEM TO BE SOLVED: To provide a method for searching for a primer set useful for sequencing a number of target genes with little cross hybridization with non-target genes. SOLUTION: The method comprises: a step in which a database is constructed from whether hybridization of the candidate primers...
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| creator | FUJIFUCHI KO CHIBA KEIWA |
| description | PROBLEM TO BE SOLVED: To provide a method for searching for a primer set useful for sequencing a number of target genes with little cross hybridization with non-target genes. SOLUTION: The method comprises: a step in which a database is constructed from whether hybridization of the candidate primers to be included in the primer set with the target genes and plural non-target genes occurs or not; a step in which the initial primer set is generated at random; and a searching step in which the following process is repeatedly conducted: the each evaluated value of the plural primer sets formed by exchanging a primer of the primer sets with plural other candidate primers are calculated based on the rates at which the primer sets hybridize with the target genes and the non-target genes, Ct and Cn, respectively; the primer sets are subjected to stochastic selection based on the calculated evaluated value; and the data of the primer set and its evaluated value are stored in the memory device. COPYRIGHT: (C)2011,JPO&INPIT |
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SOLUTION: The method comprises: a step in which a database is constructed from whether hybridization of the candidate primers to be included in the primer set with the target genes and plural non-target genes occurs or not; a step in which the initial primer set is generated at random; and a searching step in which the following process is repeatedly conducted: the each evaluated value of the plural primer sets formed by exchanging a primer of the primer sets with plural other candidate primers are calculated based on the rates at which the primer sets hybridize with the target genes and the non-target genes, Ct and Cn, respectively; the primer sets are subjected to stochastic selection based on the calculated evaluated value; and the data of the primer set and its evaluated value are stored in the memory device. 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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CALCULATING CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF COMPUTING CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES COUNTING CULTURE MEDIA ELECTRIC DIGITAL DATA PROCESSING ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | APPARATUS FOR SEARCHING PRIMER SET, METHOD AND PROGRAM FOR SEARCHING PRIMER SET |
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