APPARATUS FOR SEARCHING PRIMER SET, METHOD AND PROGRAM FOR SEARCHING PRIMER SET

PROBLEM TO BE SOLVED: To provide a method for searching for a primer set useful for sequencing a number of target genes with little cross hybridization with non-target genes. SOLUTION: The method comprises: a step in which a database is constructed from whether hybridization of the candidate primers to be included in the primer set with the target genes and plural non-target genes occurs or not; a step in which the initial primer set is generated at random; and a searching step in which the following process is repeatedly conducted: the each evaluated value of the plural primer sets formed by exchanging a primer of the primer sets with plural other candidate primers are calculated based on the rates at which the primer sets hybridize with the target genes and the non-target genes, Ct and Cn, respectively; the primer sets are subjected to stochastic selection based on the calculated evaluated value; and the data of the primer set and its evaluated value are stored in the memory device. COPYRIGHT: (C)2011,JPO&INPIT