METHOD FOR RAPIDLY AND EFFICIENTLY CAPTURING DNA FROM SAMPLE WITHOUT USING CELL LYSING REAGENT

PROBLEM TO BE SOLVED: To provide a kit for detecting K-ras mutation in biological samples.SOLUTION: There are disclosed a K-ras primer for diagnosis selected from the group consisting of an arbitrary combination of specific sequences, and a kit comprising the primer. DNA from a biological sample, fo...

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Hauptverfasser: BELLY ROBERT T, SUN JIANBO
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SUN JIANBO
description PROBLEM TO BE SOLVED: To provide a kit for detecting K-ras mutation in biological samples.SOLUTION: There are disclosed a K-ras primer for diagnosis selected from the group consisting of an arbitrary combination of specific sequences, and a kit comprising the primer. DNA from a biological sample, for example, beta-actin DNA from a serum of an individual diagnosed as a pancreatic cancer, is captured and selectively dissociated using a weakly basic homopolymer, and then the yield of amplifiable target DNA is improved by the method.
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DNA from a biological sample, for example, beta-actin DNA from a serum of an individual diagnosed as a pancreatic cancer, is captured and selectively dissociated using a weakly basic homopolymer, and then the yield of amplifiable target DNA is improved by the method.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20110127&amp;DB=EPODOC&amp;CC=JP&amp;NR=2011015691A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20110127&amp;DB=EPODOC&amp;CC=JP&amp;NR=2011015691A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BELLY ROBERT T</creatorcontrib><creatorcontrib>SUN JIANBO</creatorcontrib><title>METHOD FOR RAPIDLY AND EFFICIENTLY CAPTURING DNA FROM SAMPLE WITHOUT USING CELL LYSING REAGENT</title><description>PROBLEM TO BE SOLVED: To provide a kit for detecting K-ras mutation in biological samples.SOLUTION: There are disclosed a K-ras primer for diagnosis selected from the group consisting of an arbitrary combination of specific sequences, and a kit comprising the primer. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
title METHOD FOR RAPIDLY AND EFFICIENTLY CAPTURING DNA FROM SAMPLE WITHOUT USING CELL LYSING REAGENT
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