COMPOSITION AND METHOD FOR USING SOLID SUPPORT TO PURIFY RNA

PROBLEM TO BE SOLVED: To provide a reagent, method and kit for purification of RNA from a biological sample. SOLUTION: There are provided a formulation for isolating and purifying nucleic acids comprising a lithium salt at concentration of at least about 1 M, a surfactant, and a buffer. The inventio...

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Hauptverfasser:	MEEHAN HEATHER, WAGES JOHN M JR, PAULSEN KIM E, BAIR ROBERT J JR, HEATH ELLEN M
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a reagent, method and kit for purification of RNA from a biological sample. SOLUTION: There are provided a formulation for isolating and purifying nucleic acids comprising a lithium salt at concentration of at least about 1 M, a surfactant, and a buffer. The invention provides a formulation for isolating and purifying nucleic acids, in one embodiment, comprising at least a lithium salt at concentration of at least about 1 M, a surfactant, a buffer and if necessary a chelating agent, if necessary a reducing agent, and if necessary a tungstate, wherein the solution has a pH of above about 7. The invention further provides a method for purifying substantially pure and undegraded RNA from a biological samples containing RNAs. COPYRIGHT: (C)2011,JPO&INPIT