HOST CELL EXPRESSING RECOMBINANT HUMAN ERYTHROPOIETIN

PROBLEM TO BE SOLVED: To provide a recombinant host cell producing recombinant human erythropoietin (EPO). SOLUTION: The host cell contains a vector having a gene encoding human erythropoietin (EPO) obtained from the human genomic DNA. The gene used does not include sequences from regions at i 5...

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Hauptverfasser:	CARCAGNO CARLOS MIGUEL, MELO CARLOS, VIDAL JUAN ALEJANDRO, CRISCUOLO MARCELO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a recombinant host cell producing recombinant human erythropoietin (EPO). SOLUTION: The host cell contains a vector having a gene encoding human erythropoietin (EPO) obtained from the human genomic DNA. The gene used does not include sequences from regions at i 5' of the first translated ATG and ii 3' of the stop codon of the EPO gene. The gene has been cloned into an expression plasmid for eukaryotic cells that have the early promoter of the SV40 virus as sole expression control elements and its polyadenylation signal. The recombinant cell resulting from transfection with a used genetic construct provides an unexpectedly high level of protein expression of 50 mg of the recombinant EPO per 1 liter of culture medium per day. COPYRIGHT: (C)2010,JPO&INPIT