METHOD FOR RECOVERING VIRUS FROM CELL CULTURE USING HYPERTONIC SALT SOLUTION

PROBLEM TO BE SOLVED: To provide a method for treating a virus preparation contributing to the production of the virus preparation in high yield and purity. SOLUTION: The method for harvesting the virus from a herpesvirus-infected cultured cell is provided. The method includes treating the cultured...

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Hauptverfasser:	JOHNSTON MICHAEL DENIS, O'KEEFFE RODERIC SIMON
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for treating a virus preparation contributing to the production of the virus preparation in high yield and purity. SOLUTION: The method for harvesting the virus from a herpesvirus-infected cultured cell is provided. The method includes treating the cultured cell with a hypertonic aqueous solution, and affording a virus suspension. In the method, the production yield of the virus for live virus vaccines can be improved as compared with a method for disrupting a virus-infected cell. The harvesting step is followed by a nuclease treatment, diafiltration, and lyophilization etc. COPYRIGHT: (C)2010,JPO&INPIT