MICROPARTICLE FOR DELIVERING NUCLEIC ACID

PROBLEM TO BE SOLVED: To provide a method for delivering nucleic acid into cells, and a vector effective for delivering a polynucleotide, i.e. nucleic acid-containing microparticles having a size suitable for phagocytosis, into phagocytes without spoiling nucleic acid integrity. SOLUTION: A micropar...

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Bibliographische Detailangaben
Hauptverfasser:	LANGER ROBERT S, HEDLEY MARY LYNN, CURLEY JOANNE M, LUNSFORD LYNN B
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for delivering nucleic acid into cells, and a vector effective for delivering a polynucleotide, i.e. nucleic acid-containing microparticles having a size suitable for phagocytosis, into phagocytes without spoiling nucleic acid integrity. SOLUTION: A microparticle preparation comprising a polymer substrate and a nucleic acid expression vector is provided. The polymer substrate includes one or more synthetic polymers having solubility in water of less than about 1 mg/L. At least about 90% of the microparticles have a diameter less than about 100 micrometers. The nucleic acid is either RNA at least 50% of which is in the form of closed circles, or circular DNA plasmid molecules at least 50% of which are supercoiled. COPYRIGHT: (C)2010,JPO&INPIT