EUKARYOTIC SIGNAL SEQUENCE FOR POLYPEPTIDE EXPRESSION AND POLYPEPTIDE DISPLAY LIBRARY

PROBLEM TO BE SOLVED: To provide a method and a composition for expressing proteins or polypeptides in prokaryotic hosts by using eukaryotic signal sequences. SOLUTION: In one aspect, the present invention provides methods of expressing a Fab fragment. The methods comprise (i) a step of providing a...

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Hauptverfasser:	VEERAMALLU UDAY KUMAR, BUECHLER JOE, GRAY JEFF
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method and a composition for expressing proteins or polypeptides in prokaryotic hosts by using eukaryotic signal sequences. SOLUTION: In one aspect, the present invention provides methods of expressing a Fab fragment. The methods comprise (i) a step of providing a culture of bacterial cells containing a vector comprising a rhamnose promoter operably linked to a dicistronic transcriptional unit encoding an antibody heavy chain and an antibody light chain each operably linked to a eukaryotic signal sequence and (ii) a step of adding rhamnose to the culture to induce the rhamnose promoter whereby the antibody heavy chain and antibody light chain and their linked signal sequences are expressed, secreted to the periplasm, the signal peptide sequences are processed from the heavy and light chains, and the heavy and light chains bind to form a Fab fragment which specifically binds to a target molecule. COPYRIGHT: (C)2009,JPO&INPIT