METHOD FOR CULTURING MICROORGANISM CAPABLE OF PRODUCING COBALT-CONTAINING NITRILE HYDRATASE

PROBLEM TO BE SOLVED: To provide a culture method which aims at obtaining a microorganism having an elevated enzyme activity in efficiently producing an amide compound by using nitrile hydratase. SOLUTION: In the multistep culture of a microorganism capable of producing nitrile hydratase, an appropr...

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Bibliographische Detailangaben
Hauptverfasser:	KAZUNO YASUSHI, ARAKI TADASHI, ITO KIYOSHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a culture method which aims at obtaining a microorganism having an elevated enzyme activity in efficiently producing an amide compound by using nitrile hydratase. SOLUTION: In the multistep culture of a microorganism capable of producing nitrile hydratase, an appropriate amount of a cobalt ion species is supplied at every step in the culture to thereby elevate the enzyme activity of the microorganism thus obtained. COPYRIGHT: (C)2009,JPO&INPIT