METHOD OF DETERMINING QUANTITATIVELY BOND BETWEEN HIF-1alphaC-TERMINAL PEPTIDE AND CBP OR p300 PROTEIN, AND SCREENING METHOD FOR INHIBITOR USING THE SAME

PROBLEM TO BE SOLVED: To provide a method of determining quantitatively a bond between an HIF-1αC-terminal peptide labeled with a fluorescent substance and a CBP or p300 protein, and a screening method for an inhibitor inhibiting the formation of an HIF-1α-p300 or HIF-1α-CBP protein complex, using t...

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Hauptverfasser:	AN DAIRO, YANG EUN GYEONG, CHO KENSHU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method of determining quantitatively a bond between an HIF-1αC-terminal peptide labeled with a fluorescent substance and a CBP or p300 protein, and a screening method for an inhibitor inhibiting the formation of an HIF-1α-p300 or HIF-1α-CBP protein complex, using the method. SOLUTION: This fluorescent polarization measuring method of the present invention is useful for evaluating systematically a reaction of HIF-1α-CBP or HIF-1α-p300, and for investigating an action of modification (hydroxylation, S-nitrosation and phosphorylation) after protein synthesis of an HIF-1αC-terminal trans-active domain peptide, when replenishing the p300 protein. COPYRIGHT: (C)2008,JPO&INPIT