METHOD FOR DIAGNOSING ONYCHOMYCOSIS

PROBLEM TO BE SOLVED: To provide a diagnosis method with which a fungus causing onychomycosis can be accurately detected. SOLUTION: This method for diagnosing onychomycosis, comprising performing a first PCR and nested-PCR for a DNA extracted from a nail specimen, uses a fungus-common primer set ori...

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Hauptverfasser: TSUBOI RYOJI, EBIHARA MUTSUHITO, MAKIMURA KOUICHI
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creator TSUBOI RYOJI
EBIHARA MUTSUHITO
MAKIMURA KOUICHI
description PROBLEM TO BE SOLVED: To provide a diagnosis method with which a fungus causing onychomycosis can be accurately detected. SOLUTION: This method for diagnosing onychomycosis, comprising performing a first PCR and nested-PCR for a DNA extracted from a nail specimen, uses a fungus-common primer set originating from 28SrDNA of a fungus as the primer of the first PCR, and further using a primer set specific to 28SrDNA of a dermal fungus and a primer set specific to 28SrDNA of Scopulariosis, Aspergillus and Fusarium non-dermal fungi as the primer for the nested-PCR. COPYRIGHT: (C)2008,JPO&INPIT
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SOLUTION: This method for diagnosing onychomycosis, comprising performing a first PCR and nested-PCR for a DNA extracted from a nail specimen, uses a fungus-common primer set originating from 28SrDNA of a fungus as the primer of the first PCR, and further using a primer set specific to 28SrDNA of a dermal fungus and a primer set specific to 28SrDNA of Scopulariosis, Aspergillus and Fusarium non-dermal fungi as the primer for the nested-PCR. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title METHOD FOR DIAGNOSING ONYCHOMYCOSIS
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