IMPROVED PROTEIN DISAGGREGATION AND REFOLDING USING HIGH PRESSURE

IMPROVED PROTEIN DISAGGREGATION AND REFOLDING USING HIGH PRESSURE

PROBLEM TO BE SOLVED: To provide an improved method of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. SOLUTION: Agitation, high temperature, "stepped" depressurization, dialysis and dilution under pressure are used to increase the speed and extent...

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Bibliographische Detailangaben
Hauptverfasser: RANDOLPH THEODORE W, WEBB JONATHAN N, CARPENTER JOHN F, ST JOHN RICHARD J
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PROBLEM TO BE SOLVED: To provide an improved method of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. SOLUTION: Agitation, high temperature, "stepped" depressurization, dialysis and dilution under pressure are used to increase the speed and extent of aggregate dissolution and protein refolding. In one aspect, the method is suitably used for producing a physiologically active protein disaggregated from the protein aggregates. COPYRIGHT: (C)2008,JPO&INPIT