METHOD FOR EXPRESSING PROTEIN SITE-SPECIFICALLY INTRODUCED MODIFIED AMINO ACID

PROBLEM TO BE SOLVED: To provide a method for introducing a modified amino acid such as a phosphorylated amino acid protected with a photo-removable protective group such as bromohydroxycoumarin derivative to a specified site of a protein, and to provide tRNA usable for the introduction. SOLUTION: A...

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Hauptverfasser:	YOSHIZAKA TAKAHIRO, MURANAKA NOBUHITO, TAKAGI HIROAKI, SHIRAGAMI KAORI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for introducing a modified amino acid such as a phosphorylated amino acid protected with a photo-removable protective group such as bromohydroxycoumarin derivative to a specified site of a protein, and to provide tRNA usable for the introduction. SOLUTION: An aminoacyl tRNA containing a modified amino acid added with a protective group having bromocoumarin removable by photo-irradiation is provided. COPYRIGHT: (C)2008,JPO&INPIT