DEVICE AND METHOD FOR QUANTIFYING GENE

PROBLEM TO BE SOLVED: To enable the gene amplification by a PCR method to be simply carried out, and to enable the determination to be carried out over a wide range like a real-time PCR, and highly reliable quantitative data to be obtained. SOLUTION: PCR cycles are repeated by flowing a sample liqui...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	YONEZAWA YUJI, NAKAYAMA TAKESHI, NAKANO KOICHI, TAMIYA EIICHI, YUMINO TAKESHI, MIYOUGADANI TORU
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To enable the gene amplification by a PCR method to be simply carried out, and to enable the determination to be carried out over a wide range like a real-time PCR, and highly reliable quantitative data to be obtained. SOLUTION: PCR cycles are repeated by flowing a sample liquid in a PCR plate 3 having a denaturation temperature region A1 and an annealing temperature region A2 parallelly formed along the longitudinal direction X, and a capillary passage 2 progressing in the longitudinal direction while moving between the temperature regions A1 and A2 at both sides so as to meander, and the fluorescent intensity in each capillary passage 2 is measured so as to correspond to the number of the PCR cycles by moving an optical head 5 for measuring the fluorescent intensity. COPYRIGHT: (C)2008,JPO&INPIT