VECTOR AND METHOD FOR DETECTING CHANGE IN TRANSCRIPTIONAL LEVEL
PROBLEM TO BE SOLVED: To provide a vector simultaneously detecting effects of a test substance through activation of a transcription factor having substrate binding properties on a transcriptional activity of a gene for the plurality of transcription factors having the substrate binding properties....
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| creator | AKABOSHI HIDEKAZU YOSHIMURA SAIKO ISHIHARA MITSUKO |
| description | PROBLEM TO BE SOLVED: To provide a vector simultaneously detecting effects of a test substance through activation of a transcription factor having substrate binding properties on a transcriptional activity of a gene for the plurality of transcription factors having the substrate binding properties. SOLUTION: A method for detecting a dioxin-like activity, an estrogen-like activity or an androgen-like activity of the test substance is carried out by using a cell transformed with the vector having an enhancer region enhancing the transcriptional activity of the downstream gene in response to the test substance in a transcription control region of a TH (tyrosine hydroxylase) gene, a promoter functionally linked to the downstream side and a reporter gene functionally linked to the downstream side of the promoter. The method comprises a step of evaluation as follows. The test substance is evaluated to have the dioxin-like activity or estrogen-like activity or androgen-like activity when the expression level of the reporter gene in a cell cultured in the presence of the test substance is higher than the expression level of the reporter gene in the cell cultured in the absence of the test substance. COPYRIGHT: (C)2007,JPO&INPIT |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | VECTOR AND METHOD FOR DETECTING CHANGE IN TRANSCRIPTIONAL LEVEL |
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