VECTOR AND METHOD FOR DETECTING CHANGE IN TRANSCRIPTIONAL LEVEL

PROBLEM TO BE SOLVED: To provide a vector simultaneously detecting effects of a test substance through activation of a transcription factor having substrate binding properties on a transcriptional activity of a gene for the plurality of transcription factors having the substrate binding properties. SOLUTION: A method for detecting a dioxin-like activity, an estrogen-like activity or an androgen-like activity of the test substance is carried out by using a cell transformed with the vector having an enhancer region enhancing the transcriptional activity of the downstream gene in response to the test substance in a transcription control region of a TH (tyrosine hydroxylase) gene, a promoter functionally linked to the downstream side and a reporter gene functionally linked to the downstream side of the promoter. The method comprises a step of evaluation as follows. The test substance is evaluated to have the dioxin-like activity or estrogen-like activity or androgen-like activity when the expression level of the reporter gene in a cell cultured in the presence of the test substance is higher than the expression level of the reporter gene in the cell cultured in the absence of the test substance. COPYRIGHT: (C)2007,JPO&INPIT