SERUM-FREE MEDIUM FOR RETROVIRUS PRODUCTION

SERUM-FREE MEDIUM FOR RETROVIRUS PRODUCTION

PROBLEM TO BE SOLVED: To provide a method for acquiring a serum-free virus vector and to obtain a serum-free medium for a virus-producing cell culture usable for the method. SOLUTION: Virus-producing cells are cultured by using a serum-free medium containing serum albumin so that the cells are cultu...

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Bibliographische Detailangaben
Hauptverfasser: FUNAKOSHI MASAO, TOMURA DAISUKE, MATSUMOTO KAZUYA, TAKESAKO KAZUTADA, KATAYAMA YASUSHI, MINENO JUNICHI, KATO IKUNOSHIN, YOSHIOKA HIROFUMI
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PROBLEM TO BE SOLVED: To provide a method for acquiring a serum-free virus vector and to obtain a serum-free medium for a virus-producing cell culture usable for the method. SOLUTION: Virus-producing cells are cultured by using a serum-free medium containing serum albumin so that the cells are cultured in the same state as that of a serum-containing medium to produce a virus vector having a sufficient titer. The virus vector prepared by the virus-producing cells cultured by the medium exhibits a gene transfer efficiency equal to that of a conventional vector. The medium is useful for gene therapy and its research. COPYRIGHT: (C)2007,JPO&INPIT