FETAL RNA IN AMNIOTIC FLUID FOR DETERMINING GENE EXPRESSION IN DEVELOPING FETUS

PROBLEM TO BE SOLVED: To provide a method for determining gene expression in a developing fetus. SOLUTION: Fetal RNA is either one of the following: (a) isolated amniotic fluid fetal RNA; (b) the fetal RNA of item a, obtained by a process comprising: treating a sample of amniotic fluid obtained from...

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Hauptverfasser: JOHNSON KIRBY L, BIANCHI DAIANA W, LARRABEE PAIGE B
Format: Patent
Sprache:eng
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Zusammenfassung:PROBLEM TO BE SOLVED: To provide a method for determining gene expression in a developing fetus. SOLUTION: Fetal RNA is either one of the following: (a) isolated amniotic fluid fetal RNA; (b) the fetal RNA of item a, obtained by a process comprising: treating a sample of amniotic fluid obtained from a pregnant woman such that fetal RNA present in her amniotic fluid is extracted, resulting in amniotic fluid fetal RNA; (c) the fetal RNA of item (b), wherein cell populations are removed from the sample of amniotic fluid prior to treating the sample; and (d) the fetal RNA of item (b) wherein treating the sample of amniotic fluid comprises: isolating cell populations present in the amniotic fluid, optionally culturing the isolated cells; and extracting fetal RNA from the isolated cells. COPYRIGHT: (C)2007,JPO&INPIT