METHOD FOR EVALUATION OF BINDING BETWEEN LOW MOLECULAR WEIGHT COMPOUND WITH PROTEIN

PROBLEM TO BE SOLVED: To provide a method for evaluation of bonding between a low molecular weight compound with a protein which extrudes detection of the bonded substance itself from the target of detection. SOLUTION: The invention relates to the method for evaluation of bonding between the low molecular weight compound with the protein comprising (1) a process preparing a first DNA having a protruding end and the low molecular weight compound fixed on the protruding end, (2) a process preparing a second DNA having a protruding end bondable with the protruding end of the first DNA, (3) a process making the first DNA and the second DNA coexist under the presence of a protein and a DNA ligase, and (4) a process detecting a signal DNA comprising the first DNA bonded with the second DNA. Problems of conventional method for evaluation of bonding between low molecular weight compound with protein that the conventional method was detecting the bonding substance itself and requires that the bonding substance have to be kept in a specific environment and the method is hard to evaluate the bonding caused by the effect of the environment. COPYRIGHT: (C)2007,JPO&INPIT