CHIMERIC PROTEIN OF TISSUE FACTOR ACTIVITY INHIBITOR, TFPI AND TFPI-2, AND MUTEIN

PROBLEM TO BE SOLVED: To provide a method of efficiently producing a protein molecule with less glycosylated sites, which has anticoagulant activity at least equal to that of TFPI and other activities when not reinforced, and which reduces immunogenicity of the protein when administered to a mammal....

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Hauptverfasser:	INNIS MICHAEL A, CREASEY ALBA A
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method of efficiently producing a protein molecule with less glycosylated sites, which has anticoagulant activity at least equal to that of TFPI and other activities when not reinforced, and which reduces immunogenicity of the protein when administered to a mammal. SOLUTION: The method is to produce chimeric proteins which contain (a) Kunitz-type domain 1 of TFPI-2 and (b) Kunitz-type domain 2 of TFPI, which includes (a) process of providing transformed host cells including expression vectors with polynucleotide sequences encoding the chimeric proteins, further with expression-controlling sequences compatible with expression of the sequences of the polynucleotide sequences encoding the chimeric proteins, and (b) process of incubating the transformed host cells under a condition that the polynucleotide sequences encoding the chimeric proteins can be expressed. COPYRIGHT: (C)2006,JPO&NCIPI