NEW beta-N-ACETYLHEXOSAMINIDASE

PROBLEM TO BE SOLVED: To obtain an enzyme useful for producing N-acetylglucosamine from a chitin oligosaccharide. SOLUTION: A protein (enzyme) having the following physicochemical properties. (a) Action: the protein catalyzes an enzymatic reaction for hydrolyzing an oligosaccharide to produce N-acet...

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Hauptverfasser:	UEDA KENJI, OGAWA SHINKO, BEPPU TERUHIKO, OKU TADATAKE, NISHIO TOSHIYUKI, KAWACHI TAKASHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To obtain an enzyme useful for producing N-acetylglucosamine from a chitin oligosaccharide. SOLUTION: A protein (enzyme) having the following physicochemical properties. (a) Action: the protein catalyzes an enzymatic reaction for hydrolyzing an oligosaccharide to produce N-acetylglucosamine. (b) Molecular weight: 55,800±500. (c) Optimal pH and stable pH range: the optimal pH is 5.5, when pNP N-acetyl-β-D-glucosaminide is used as a substrate, and the stable pH range is 5.3 to 10. (d) The range of a proper action temperature: a range of 55 to 75°C. (e) Thermal stability: when the protein is treated at pH 5.5 for 30 minutes, the protein holds the activity of ≥90% at 55°C, and the activity of 60% at 60°C. COPYRIGHT: (C)2006,JPO&NCIPI