METHOD FOR EXTRACTING RNA, REAGENT FOR EXTRACTING RNA, AND METHOD FOR ANALYZING BIOMATERIAL

PROBLEM TO BE SOLVED: To provide a method for extracting an RNA with high purity and analyzing it through safe, rapid and simple operation from a biomaterial containing the RNA. SOLUTION: The method comprises adding a chaotropic agent at a predetermined concentration and an organic solvent at a pred...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	UCHIDA NORITAKA, YAMASHITA YOSHIHIRO, KUNO NORITO, SAKURAI TOMOYA, YOKOBAYASHI TOSHIAKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for extracting an RNA with high purity and analyzing it through safe, rapid and simple operation from a biomaterial containing the RNA. SOLUTION: The method comprises adding a chaotropic agent at a predetermined concentration and an organic solvent at a predetermined concentration, mixing them, making a nucleic acid-binding solid phase contact to the mixed liquid, washing the nucleic acid-binding solid phase bound with the RNA and eluting the RNA from the nucleic acid-binding solid phase bound with the RNA. The obtained RNA is also analyzed by reverse transcription polymerase chain reaction (RT-PCR) and so on. COPYRIGHT: (C)2006,JPO&NCIPI