METHOD FOR NECROCYTOSIS AND DEVICE THEREFOR

PROBLEM TO BE SOLVED: To provide a necrocytosis method and necrocytosis device to efficiently and surely necrotize a target cell. SOLUTION: The necrocytosis method is composed of a specimen holding step to place a specimen cell on a microscope, a cell image acquiring step to acquire a digital image of the specimen, a cell image processing step to determine the laser radiation position, and a laser radiation step to radiate laser light to the specimen. The cell image processing step is composed of a step to determine the presence of a cell in a pixel for each pixel of the image and binarize the image data, a step to find the boundary of two adjacent pixels having different values in the binarized image data and find an isolated closed curve, a step to process the binarized image data under the assumption of the presence of cells in the whole inner side of the isolated closed curve, and a step to divide the binarized and processed image data into regions of predetermined size, calculate the gravity center coordinates of the region recognized to hold the cell and setting the gravity center position as the laser radiation position. COPYRIGHT: (C)2006,JPO&NCIPI