PRIMER PANEL FOR MULTIPLEX PCR, MULTIPLEX PCR METHOD USING THE SAME, AND GENE ANALYZING METHOD

PROBLEM TO BE SOLVED: To provide a primer panel which is used for multiplex PCR (polymerace chain reaction), can surely perform the multiplex PCR, and is designed to facilitate the interpretation of separation data, to provide a multiplex PCR method, and to provide a gene-analyzing method. SOLUTION: This primer panel is used for the multiplex PCR, contains a primer pair, and is designed to have two to twenty specific zones which are amplified and whose at least one is the positive control of PCR amplification, and to give amplification PCR products having chain lengths of 50 to 600 bp. The primer pair can PCR-amplify a specific domain on a chromosome. Either primer of the primer pair is complementary with one end of the specific domain, and the other of the specific domain is complementary with the other end of the sequence. The primers have chain lengths of 15 to 25 bp, GC (guanorine and cyticline) contents of 30 to 60 %, Tm values of 52 to 72, and 3' terminals of G or C, respectively. COPYRIGHT: (C)2005,JPO&NCIPI