METHOD FOR INDUSTRIAL CRYSTALLIZATION OF TRANSGLUTAMINASE AND METHOD FOR PRODUCING TRANSGLUTAMINASE PREPARATION
PROBLEM TO BE SOLVED: To provide an industrial method for easily crystallizing a transglutaminase produced by microorganisms of the genus Streptomyces and a method for producing a transglutaminase preparation by including an additive in the transglutaminase crystallized by the crystallization method...
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| creator | WASHIZU KINYA KURONO YOSHIAKI |
| description | PROBLEM TO BE SOLVED: To provide an industrial method for easily crystallizing a transglutaminase produced by microorganisms of the genus Streptomyces and a method for producing a transglutaminase preparation by including an additive in the transglutaminase crystallized by the crystallization method. SOLUTION: The method for the industrial crystallization of transglutaminase comprises the addition of sodium chloride and/or potassium chloride to crude transglutaminase produced by microorganisms of the genus Streptomyces or to a partially purified enzyme to perform the salting-out of the enzyme. The addition of the sodium chloride and/or potassium chloride may be carried out slowly to the saturated amount. Streptomyces mobaraensis or Streptomyces lavendulae may be used as the microorganism of the genus Streptomyces. COPYRIGHT: (C)2005,JPO&NCIPI |
| format | Patent |
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SOLUTION: The method for the industrial crystallization of transglutaminase comprises the addition of sodium chloride and/or potassium chloride to crude transglutaminase produced by microorganisms of the genus Streptomyces or to a partially purified enzyme to perform the salting-out of the enzyme. The addition of the sodium chloride and/or potassium chloride may be carried out slowly to the saturated amount. Streptomyces mobaraensis or Streptomyces lavendulae may be used as the microorganism of the genus Streptomyces. 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SOLUTION: The method for the industrial crystallization of transglutaminase comprises the addition of sodium chloride and/or potassium chloride to crude transglutaminase produced by microorganisms of the genus Streptomyces or to a partially purified enzyme to perform the salting-out of the enzyme. The addition of the sodium chloride and/or potassium chloride may be carried out slowly to the saturated amount. Streptomyces mobaraensis or Streptomyces lavendulae may be used as the microorganism of the genus Streptomyces. 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SOLUTION: The method for the industrial crystallization of transglutaminase comprises the addition of sodium chloride and/or potassium chloride to crude transglutaminase produced by microorganisms of the genus Streptomyces or to a partially purified enzyme to perform the salting-out of the enzyme. The addition of the sodium chloride and/or potassium chloride may be carried out slowly to the saturated amount. Streptomyces mobaraensis or Streptomyces lavendulae may be used as the microorganism of the genus Streptomyces. COPYRIGHT: (C)2005,JPO&NCIPI</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD FOR INDUSTRIAL CRYSTALLIZATION OF TRANSGLUTAMINASE AND METHOD FOR PRODUCING TRANSGLUTAMINASE PREPARATION |
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