METHOD FOR HYBRIDIZATION, METHOD FOR DETECTING BASE SEQUENCE AND KIT FOR DETECTING BASE SEQUENCE

PROBLEM TO BE SOLVED: To provide a method for hybridization with which the hybridization of a probe with a target base sequence can be carried out with high selectivity and accuracy even when the concentration of the target base sequence is ultralow. SOLUTION: The method for the hybridization is cha...

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Bibliographische Detailangaben
1. Verfasser:	MASUKO MASAYUKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for hybridization with which the hybridization of a probe with a target base sequence can be carried out with high selectivity and accuracy even when the concentration of the target base sequence is ultralow. SOLUTION: The method for the hybridization is characterized in that a peptide nucleic acid probe immobilized on a solid-phase support is brought into contact with a solution containing a target nucleic acid having a base sequence complementary to the peptide nucleic acid probe and further containing a hydrophilic organic solvent in an amount of 1.0-3.0 mol/L based on the solution and regulated to pH 9.1-10.6. Thereby, the peptide nucleic acid probe is hybridized with the target nucleic acid. COPYRIGHT: (C)2005,JPO&NCIPI