PROCESS FOR IMMOBILIZING ENZYMES ON CELL WALL OF MICROBIAL CELL BY PRODUCING FUSION PROTEIN

PROBLEM TO BE SOLVED: To provide a method for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. SOLUTION: The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokariotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale. COPYRIGHT: (C)2004,JPO&NCIPI